Malcolm Whiteway, PhD
Professor and Canada Research Chair in Microbial Genomics, Biology
PhD (University of Alberta)
Our ongoing research investigates the medically important fungal pathogen Candida albicans, with the ultimate goal of identifying new treatment options for patients suffering from Candida infections. We use an array of genetic and genomic approaches to investigate pathways involved in various C. albicans functions. As part of these efforts we collaborated with various international research teams in the assembly and annotation of the C. albicans genome. We are now using genome-based tools such as transcriptional profiling and ChIP-chip analysis to investigate regulatory circuits in the pathogen. We have identified dramatic cases of transcriptional rewiring of central metabolic pathways, synchronized cells and established the transcriptional profile of the C. albicans cell cycle, and investigated the regulation of the fungal mating system. We are also screening an extensive library of conditional C. albicans mutants using various physiological and chemical treatments through the functional Genomics robotic facility at Concordia. This will allow us to explore, tailor, and understand the subtleties that make C. albicans a human pathogen.
NSERC, FQRNT, Genome Quebec, Canada Research Chairs
BIOL 511/BIOL 481, Structural Genomics
BIOL 261, Genetics
Feng J, Islam A, Bean B, Feng J, Sparapani S, Shrivastava M, Goyal A, Omran, RP, Mallick J, Whiteway M. (2020) Hof1 plays a checkpoint-related role in MMS-induced DNA damage response in Candida albicans. Mol Biol Cell, 31: 348-359.
Feng J, Yao S, Dong Y, Hu J, Whiteway M, Feng J. Nucleotide excision repair protein Rad23 regulates cell virulence independent of Rad4 in Candida albicans (2020) mSphere 5:e00062-20
Xu H, Fang T, Omran RP, Whiteway M, Jiang L (2020) RNA sequencing reveals an additional Crz1-binding motif in promoters of its target genes in the human fungal pathogen Candida albicans. Cell Commun Signal. 18:1.
Costa ACBP, Omran RP, Correia-Mesquita TO, Dumeaux V, Whiteway M. (2019) Screening of Candida albicans GRACE library revealed a unique pattern of biofilm formation under repression of the essential gene ILS1.Sci Rep. 9: 9187.
Rastghalam G, Omran RP, Alizadeh M, Fulton D, Mallick J, Whiteway M. (2019) MAP Kinase Regulation of the Candida albicans Pheromone Pathway. mSphere. 4: e00598-18.
Sharmeen N, Sulea T, Whiteway M, Wu C. (2019) Adapter protein Ste50p directly modulates yeast MAPK signaling specificity through differential connections of its RA domain. Mol Biol Cell. 30: 794-807.
Polvi EJ, Veri AO, Liu Z, Hossain S, Hyde S, Kim SH, Tebbji F, Sellam A, Todd RT, Xie JL, Lin ZY, Wong CJ, Shapiro RS, Whiteway M, Robbins N, Gingras AC, Selmecki A, Cowen LE. (2019) Functional divergence of a global regulatory complex governing fungal filamentation. PLoS Genet. 15:e1007901.
Islam A, Tebbji F, Mallick J, Regan H, Dumeaux V, Omran RP, Whiteway M. (2018) Mms21: A Putative SUMO E3 Ligase in Candida albicans That Negatively Regulates Invasiveness and Filamentation, and Is Required for Genotoxic and Cellular Stress Response.
Genetics. 211: 579-595.
Xu H, Whiteway M, Jiang L. (2018) The tricarboxylic acid cycle, cell wall integrity pathway, cytokinesis and intracellular pH homeostasis are involved in the sensitivity of Candida albicans cells to high levels of extracellular calcium. Genomics. S0888-7543(18)30307-0.
Shahina Z, El-Ganiny AM, Minion J, Whiteway M, Sultana T, Dahms TES. (2018) Cinnamomum zeylanicum bark essential oil induces cell wall remodelling and spindle defects in Candida albicans. Fungal biology and biotechnology 5, 3.
Veri AO, Miao Z, Shapiro RS, Tebbji F, O'Meara TR, Kim SH, Colazo J, Tan K, Vyas VK, Whiteway M, Robbins N, Wong KH, Cowen LE. (2018) Tuning Hsf1 levels drives distinct fungal morphogenetic programs with depletion impairing Hsp90 function and overexpression expanding the target space. PLoS Genetics 14, e1007270
Chen Y, Mallick J, Maqnas A, Sun Y, Choudhury BI, Cote P, Yan L, Ni TJ, Li Y, Zhang D, Rodríguez-Ortiz R, Lv QZ, Jiang YY, Whiteway M. (2018) Chemogenomic profiling of the fungal pathogen Candida albicans. Antimicrob Agents Chemother. 62, e02365
Tebung WA, Omran RP, Fulton DL, Morschhäuser J, Whiteway M. (2017) Put3 Positively Regulates Proline Utilization in Candida albicans. mSphere. 2, e00354-17.
Scaduto CM, Kabrawala S, Thomson GJ, Scheving W, Ly A, Anderson MZ, Whiteway M, Bennett RJ. (2017) Epigenetic control of pheromone MAPK signaling determines sexual fecundity in Candida albicans. Proc Natl Acad Sci U S A. 114, 13780-85.
Tebbji F, Chen Y, Sellam A, Whiteway M. (2017) The Genomic Landscape of the Fungus-Specific SWI/SNF Complex Subunit, Snf6, in Candida albicans. mSphere 2, e00497-17.
Regan H, Scaduto CM, Hirakawa MP, Gunsalus K, Correia-Mesquita TO, Sun Y, Chen Y, Kumamoto CA, Bennett RJ, Whiteway M. (2017) Negative regulation of filamentous growth in Candida albicans by Dig1p. Mol Microbiol. 105, 810-824.
Shekhar-Guturja T, Tebung WA, Mount H, Liu N, Köhler JR, Whiteway M, Cowen LE. Beauvericin Potentiates Azole Activity via Inhibition of Multidrug Efflux, Blocks Candida albicans Morphogenesis, and Is Effluxed via Yor1 and Circuitry Controlled by Zcf29. Antimicrob Agents Chemother. 60, 7468-7480.
Tebung, WA, Choudhury, BI, Tebbji, F, Morschhäuser, J, Whiteway, M. (2016) In the Saccharomycetaceae the Ppr1 zinc cluster transcription factor has been rewired from purine catabolism to pyrimidine biogenesis. Curr. Biol 26:1677-87.
Sun Y, Gadoury C, Hirakawa MP, Bennett RJ, Harcus D, Marcil A, Whiteway M. (2016) Deletion of a Yci1 Domain Protein of Candida albicans Allows Homothallic Mating in MTL Heterozygous Cells. mBio. 7, e00465-16.
Zhao Y, Yan H, Happeck R, Peiter-Volk T, Xu H, Zhang Y, Peiter E, van Oostende Triplet C, Whiteway M, Jiang L. (2016) The plasma membrane protein Rch1 is a negative regulator of cytosolic calcium homeostasis and positively regulated by the calcium/calcineurin signaling pathway in budding yeast. Eur J Cell Biol. 95, 164-74
Mallick J, Jansen G, Wu C, Whiteway M. (2016) SRYTH: A New Yeast Two-Hybrid Method. Methods Mol Biol. 1356, 31-41.
Yan L, Xiong J, Lu H, Lv QZ, Ma QY, Côte P, Whiteway M, Jiang YY (2015) The Role of Mms22p in DNA Damage Response in Candida albicans (2015) G3 (Bethesda). 5, 2567-78.