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http://www.concordia.ca/content/concordia/en/research/microscopy-cellular-imaging/using-the-centre.html

Using the Centre

How to prepare for your visit

To collect proper data from imaging, you need to prepare ahead. Ask yourself:  

  • What is the scientific question you want to answer? Where is your signal located in the cells?
  • What controls are needed to distinguish a meaningful experimental result?
  • What is the resolution you need? How many dimensions? (x, y, z, t, multi-color)
  • Do you need to fix your samples or use live specimens?
  • If live imaging is required, what are you expecting in terms of movements? For how long will you need to image? At what frame rate? Do you need environmental control?
  • Before considering which dye or fluorescent probes to use, or how to prepare your samples, visit our website or contact us to check the compatibility of your experimental design and needs with the equipment offered by the Centre.  

Our policies

The goal of the CMCI is to get users high quality results in as little time possible. These policies are in place so that our users can make the most of our facility and can use the equipment properly and efficiently.

 

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