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Wide-field fluorescent microscopes

Leica DMI6000B inverted epifluorescence microscope

 

The Leica DMI 6000B is an automated inverted microscope outfitted with a controllable X-cite mercury lamp and an extensive collection of filter cubes (360, 488, 560, 604 nm excitation) for fluorescent microscopy, and a halogen lamp for brightfield and DIC. It is equipped with 4 bright lenses (10, 20, 40, 63x), a high resolution Hamamatsu Orca R2 CCD camera (1344x1024 pixels), a motorized stage (XY only) and an environmental chamber will be available soon for live cell imaging. The system is operated using PerkinElmer Volocity software.


Leica DM6000B epifluorescence microscope

 

The Leica DM6000B is an semi-automated upright microscope outfitted with a mercury lamp and an extensive collection of filter cubes for fluorescent microscopy, and a halogen lamp for brightfield and DIC. It is equipped with 4 lenses, a high resolution Hamamatsu Orca ER CCD camera, and a motorized stage (XY only) optimized for imaging live yeast cells and fixed mammalian cells. The system is operated using PerkinElmer Volocity software.
 


Nikon Eclipse TiE inverted epifluorescence microscope

The Nikon Eclipse TiE is a fully automated inverted microscope outfitted with two light sources for fluorescence imaging: a Heliphor LED-based source (405, 480, 555, 640 nm) to limit phototoxicity and a super bright, wide spectrum Sutter Lamda XL source with a collection of filter cubes for intracellular ion measurement. Two cameras are mounted on this sytem: a Photometrics Evolve 512 EMCCD for low light or single molecule applications and a Nikon high resolution (4908 x 3264 pixels) color camera DsRi2 CMOS. A Photometrics CoolSNAP KINO CCD for high resolution live cell imaging is also available upon request. It is equipped with five bright lenses (4, 10, 40, 60, 100x), DIC optics, a motorized stage (including piezo Z control) and a gas- and temperature- controlled stagetop incubator. The system is operated using NIS-Elements software.

 


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