Leica TCS SP2 Confocal Microscope
The Leica TCS SP2 system is equipped to provide brightfield, differential interference contrast and epi-fluorescent visualization of microscopic specimens. The onboard optic components include 10x, 20x (IMM CORR), 40x (OIL), 63x (OIL) and 100x (OIL) objectives and green and red long-pass epi-fluorescence filter cubes. A 50W mercury vapor lamp supplies illumination for epi-fluorescent excitation. The laser scanner supplies coherent visible light at the following wavelengths: 458 nm, 476 nm, 488 nm, 496 nm, 543 nm and 633 nm. These spectral lines are capable of exciting a broad range of fluorophores including: fluorescein, Oregon Green, rhodamine, Cy3, Texas Red, Cy5 as well as fluorescent protein derivatives (GFP, CFP, YFP, RFP, dsRed). Leica's patented spectrophotometric acquisition system permits the operator unlimited flexibility to adjust the spectral characteristics of the fluorescence emission detectors in real time.
Precise positioning of pinhole aperatures at points within the optical train of the confocal microscope excludes "out of focus light" emanating from fluorescent structures that lie outside of the focal plane of the specimen. This enables the digital acquisition of clean optical sections whose details are not obscured by objects located above and below the focal plane. By moving the focal plane axially through a specimen, a series of optical sections can be acquired and stacked together in order to render a 3-dimensional representation of its fluorescently labelled structures.
With an optical resolution of 200 - 300 nm, confocal microscopy can be applied to determine where fluorescently labelled macromolecules are being localized on the sub-cellular level. It can also be used to assess the co-localization of multiple gene products as well as tracking the movement of intracellular structures in vivo. The Leica Confocal Acquisition Software includes application tools for performing a variety of advanced photobleaching techniques in vivo including: Fluorescent Resonance Energy Transfer (FRET), Fluorescence Recovery After Photobleaching (FRAP), and Fluorescence Loss in Photobleaching (FLIP). FRET can be applied to study protein-protein interactions while FRAP and FLIP are useful for looking at molecular diffusion kinetics within selected sub-cellular compartments.
Location: GE S105. Currently administered by the Center for Microscopy at Concordia.
Zeiss Axioplan Fluorescence Microscope
The Zeiss Axioplan fluorescence microscope is mounted with a Lumenera Infinity 3-1C 1.4 megapixel color cooled CCD camera and can be used to perform compound digital micrography at the CSFG. The microscope is equipped with 10x, 40x (DRY), 63x (OIL), 63x (OIL DIC) and 100x (OIL) objectives and other optic components required to perform brightfield, phase contrast and differential interference contrast microscopy. Illumination for epi-fluorescent excitation is provided an X-cite 120Q illuminator from EXFO.
Filter set A:
UV Filter Cube #487902 (Exciter Filter: G 365, Beam Splitter: FT 395, Barrier Filter: LP 420). This cube is used to visualize UV-excitable fluors like DAPI.
Blue Filter Cube #487910 (Exciter Filter: BP 450-490, Beam Splitter: FT 510, Barrier Filter: BP 515-565). Fluorescein, GFP and Oregon Green can be visualized using this filter cube.
Green Filter Cube #487915 (Exciter Filter: BP 546±12, Beam Splitter: FT 580,
Barrier Filter: LP 590). Green-excitable fluors like rhodamine and Cy3 can be visualized using this filter cube.
Filter set B:
GFP Filter Cube #1031346 (Exciter Filter: BP 470/40, Beam Splitter: FT 495, Barrier Filter: BP 525/30). This cube is used to visualize eGFP, CFP, GFP (S65T).
dsRed Filter Cube #1114462 (Exciter Filter: BP 560/40, Beam Splitter: FT 585, Barrier Filter: BP 630/75). This cube is used to visualize dsRed and mRFP.
YFP Filter Cube #1196681 (Exciter Filter: BP 500/20, Beam Splitter: FT 515,
Barrier Filter: BP 535/30). This cube is used to visualize eYFP, eGFP and Alexa 488.
Location: GE S105.
Computer workstations in this room are made available to users for data acquisition, for data analysis and for teaching-related purposes. In addition to the Leica Confocal Acquisition Software and the Genepix microarray data analysis software, a number of other software have been installed for data analysis.
Location: GE S101.
Singer MSM Micromanipulator
The micromanipulator is primarily used for spore dissection of sporulating yeasts. Using a microscope for visualization (4x and 20x objective lenses) and a highly precise movable stage (resolution of 4 µm and repeatability of 2 µm) and an extremely thin tapered glass capillary needle (40 - 50 µm diameter) for manipulation, spores can be separated and placed on pre-determined sections of a solid growth-medium plate. In such a manner, strains with different genotypes can be isolated.
Location: SP 444.01
Nikon SMZ1500 Stereomicroscope
The Nikon SMZ1500 is a stereomicroscope with a 15:1 zoom ratio and a 0.75X to 11.25X zoom range. A 10X eyepiece allows total magnification of 7.5X to 112.5X. Illumination is provided by a 30W halogen lamp in the base and/or by an external 150W fiber optic episcopic illuminator that can provide bright illumination over the entire surface of the sample. A beam splitter allows images to be captured on a Leica DFC420 5 megapixel color digital camera.
Location: GE S105.
Nikon TMS Inverted Microscope
The Nikon TMS is an inverted microscope basic phase contrast capability (10x, 20x and 40x). Illumination is provided by a 20W halogen lamp in the top of the microscope. A beam splitter allows images to be captured on a SPOT Insight 2 megapixel color digital camera.
Location: GE S110-01. Access restricted to users of this facility.