Concordia University

http://www.concordia.ca/content/concordia/en/research/genomics/facilities-instruments/analytical.html

Analytical

Mass Spectrometry
Liquid Chromatography
Other

Thermo Finnigan FT-ICR-MS
GE S107

Dionex ICS-5000 Ion Chromatography System
GE 220.01

Accuri C6 flow cytometer
GE 320.01

Thermo LTQ MS with LDTD
GE S103 (Martin)

Amersham Pharmacia Biotech Akta Purifier
GE S103

Beckman Optima XL-I Analytical Ultracentrifuge
GE 109

Thermo LTQ MS with 2D LC
GE S103 (Martin)

BioRad BioLogic DuoFlow HPLC
GE 200.00

Microcal VP-ITC Microcalorimeter
SP 280.18

Agilent GC-MS
GE S103 (Martin, Tsang, Titorenko)

Agilent 1200 series HPLC
GE 320.00 (Martin)

 
 

Thermo Surveyor HPLC
GE 320.00 (Martin)

 
 

HPLC Pressure Cell
GE 320.00 (Martin)

 

Thermo Finnigan FT-ICR-MS (Fourier Transform Ion Cyclotron Resonance Mass Spectrometer)

Thermo Finnigan FT-ICR-MS

The Thermo Finnigan Fourier Transform Ion Cyclotron Resonance Mass Spectrometer is coupled to a HPLC and ESI source. It is a high resolution mass spectrometer intended for proteomics and metabolomics applications with the following specifications:

  • 7 Tesla field magnet
  • Attomole sensitivity
  • Dynamic range : 50 to 4000Da
  • Mass accuracy : <1.2ppm RMS error with external calibration; <1ppm RMS error with internal calibration
  • Resolution : 100,000 at m/z 400 at 1 sec. per scan; >750,000 at m/z 400 at slower scan repetition rates

Kindly donated by Merck.

Location: GE S107


Thermo LTQ MS with LDTD
(Linear Ion Trap Quadrupole Mass Spectrometer with Laser Diode Thermal Desorption)

Thermo LTQ Orbitrap MS with LDTD

Linear Ion Trap Quadrupole Mass Spectrometer with Laser Diode Thermal Desorption ion source.

The Phytronix LDTD source allows for high throughput analysis in 96 and 384 well-plate format.

Description to be provided.

Location: GE S103 (Martin lab)


Thermo LTQ MS with 2D LC
(Linear Ion Trap Quadrupole Mass Spectrometer with Dual Column Liquid Chromatography)

Thermo LTQ Orbitrap MS with 2D LC

Linear Ion Trap Quadrupole Mass Spectrometer with Dual Column Liquid Chromatography and ESI.

Description to be provided.

Location: GE S103 (Martin lab)


Agilent GC-MS (Gas Chromatographer with Mass Spectrometer)

Agilent GC-MS

Location: GE S103 (Martin, Titorenko and Tsang)


Dionex ICS-5000 Ion Chromatography System

Dionex ICS-3000 Ion Chromatography System

The Dionex ICS-5000 is a HPAEC-PAD (High Performance Anion Exchange Chromatography - Pulsed Amperometric Detection) system for the separation and detection of sugars and oligosaccharides, including modifications such as uronic acids and sugar alcohols. It uses high pH eluents to ionize sugars and gold electrodes to oxidize them, giving better separation and sensitivity than other carbohydrate HPLC systems. It is equipped with an autosampler, isocratic or gradient separation, fraction collector and Chromeleon software for data collection and analysis.

Location: GE 220.01


Amersham Pharmacia Biotech Akta Purifier

Amersham Pharmacia Biotech Akta Purifier

The AKTApurifier 10 is a high performance liquid chromatography system capable of purifying and characterizing peptides and nucleic acids. It can run any technique and is able to perform scouting, method optimization and buffer preparation. The system can purify ug to mg quantities at flow rates of 0.001 to 10 ml/min at pressures of 0 to 25 MPa. It can monitor pH from 2 - 12, conductivity from 1 uS/cm to 999.9 mS/cm and absorbance from 190 - 700 nm (in 1 nm steps) using up to three different wavelengths simultaneously. An X/Y fraction collector distributes flow through samples into 18 mm tubes (Rack A: 120 x 18 mm tubes plus 8 x 30 mm tubes), 12 mm tubes (Rack B: 240 x 12 mm tubes) or 96-well microtiter plates (Rack C: 4 microtiter plates plus 8 x 30 mm tubes). The unit owned by the CSFG has increased flexibility since it is equipped with additional valves for multiple sample injection, column manifolding and multiple buffer selection.

Location: GE S105


BioRad BioLogic DuoFlow HPLC
BioRad BioLogic DuoFlow HPLC

Location: SP 280.18


Agilent 1200 series HPLC

Agilent 1200 series HPLC

Location: SP 280.18


Thermo Surveyor HPLC

Thermo Surveyor HPLC

Location: SP 280.18


HPLC Pressure Cell

Location: SP 280.18


Accuri C6

Accuri C6

Location: SP 280.18


Beckman Optima XL-I Analytical Ultracentrifuge

Beckman Optima XL-I Analytical Ultracentrifuge

The XL-I analytical ultracentrifuge is used to study the behaviour of proteins in solution. The purity of the protein sample, the state of the protein in different solutions or at different temperatures (0° - 40° C), and whether a specific protein interacts with macromolecules, such as DNA, other proteins or specific drugs, can all be monitored. In addition, a number of different physical parameters of the protein of interest can be calculated. The sedimentation of the protein samples as they are being subjected to centrifugal force is monitored using either absorbance or Rayleigh interference optics; absorbance can be measured from 190 - 800 nm, and the interference laser functions at 675 nm. The interference optics provide a much greater capability to the instrument than the standard absorbance optics alone, since molecules which do not possess significant absorbance, such as polysaccharides, or solutions which have a very high absorbance (for example, if they contain ATP or tyrosine) can also be studied. The An-60 Ti analytical rotor can accomodate up to 3 cells for a total of 3 samples during sedimentation velocity runs or 9 samples during sedimentation equilibrium runs. Either quartz (for absorbance optics) and sapphire (for interference optics) windows can be assembled with the cells.

Location: GE 109


Microcal VP-ITC Microcalorimeter

Microcal VP-ITC Microcalorimeter

The VP-ITC isothermal titration microcalorimeter measures the heat that is generated or absorbed in liquid samples as a result of mixing two or more reactants. This is a highly precise instrument that can detect changes to just above one nanocalorie per second and can operate from 2° - 80° C. It can be used to study the interaction of proteins to other proteins, DNA or other ligands. The data that is generated can be used to calculate various physical parameters, such as the reaction stoichiometry, binding constant, enthalpy and entropy.

Location: SP 280.18

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