LTQ Orbitrap Velos with ETD (Thermo Fisher Scientific)
Applications: Protein analysis (bottom-up proteomics, top-down proteomics, post translational modification (PTM) analysis and relative quantitation); Metabolite analysis (untargeted metabolomics and metabolite structural elucidation); Lipid analysis (untargeted lipidomics, shotgun lipidomics). Click here to see more.
Ionization Sources: Heated electrospray ionization (H-ESI) and nanospray ESI (NSI)
Sample Introduction: HPLC (Agilent 1100), nanoLC (Thermo EASY nLC II) and LDTD (Phytronix)
Mass Analyzer: Orbitrap & linear ion trap MS
Mass Range: m/z 50-2,000, m/z 200-4000
Resolution: 60,000 at m/z 400 at a scan rate of 1 Hz, maximum resolution >100,000 at m/z 400
Accuracy: <3 ppm with external calibration, <1 ppm using internal calibration
ETD (electron transfer dissociation), generates complementary information for highly sensitive PTM analysis and peptide de novo sequencing.
LDTD (laser diode thermal desorption), direct sample introduction ion source for high-throughput analysis of metabolites.