Workshops & seminars

The chemistry of breathing: Wrinkle-to-fold transitions in lung surfactants and other elastic sheet
Dr. Ka Yee Lee(University of Chicago)

Friday, November 10, 2017
2:45 p.m. – 4 p.m.

Dr. Ka Yee Lee


This event is free





Hingston Hall, wing HC
7141 Sherbrooke W.
Room HC-157



AbstractLung surfactant is a mixture of lipids and proteins that coats the alveoli, and its main mechanical function is to reduce the work of breathing by reducing the surface tension. Insufficient amount of lung surfactant in premature infants leads to neonatal respiratory distress syndrome, while lung trauma can result in acute respiratory distress syndrome. In order to develop effective treatment for these conditions, a better understanding of the interactions between lung surfactant lipids and proteins is needed.  Utilizing optical and atomic force microscopy techniques, we have examined the collapse process in lung surfactant, and have examined how the presence of lung surfactant peptide, SP-B1-25, induces a reversible collapse in lung surfactant monolayers. Our observation indicates that SP-B1-25 in simple phospholipid and model lung surfactant monolayers promote the protrusion of folds into the subphase at low surface tensions.  The folds remain attached to the monolayer and reversibly reincorporated upon expansion.  Without SP-B, an unsaturated lipid-rich phase is irreversibly "squeezed-out" of the monolayer at higher surface tensions.  These folded reservoirs reconcile how lung surfactant can achieve both low surface tensions upon compression and rapid respreading upon expansion, and have important implications concerning the design of replacement lung surfactants. The onset of this folding instability can be understood in terms of the mechanical properties of the film. Statistics of the folding events will be presented and the link between folding on monolayers of nm thickness and that on polyester films that are 3 orders of magnitude thicker will be discussed. By studying different types of monolayers, we have shown that this folding transition in monolayers is not limited to lung surfactant films, but rather represents a much more general type of stress relaxation mechanism. Our study indicates that collapse modes are found most closely linked to in-plane rigidity. We characterize the rigidity of the monolayer by analyzing in-plane morphology on numerous length scales. More rigid monolayers collapse out-of-plane via a hard elastic mode similar to an elastic membrane, with the folded state being the final collapse state, while softer monolayers relax in-plane by shearing. For the hard elastic mode of collapse, we have further demonstrated experimentally and theoretically that the folded state is preceded by a wrinkled state.

Recent developments in the chemistry of corrole macrocycles

Bio:  Ka Yee C. Lee, Professor of the University of Chicago Chemistry Department, the James Franck Institute, Institute for Biophysical Dynamics and the College, also serves as the Senior Associate Vice President for Research. She leads efforts in the Office of the Executive Vice President for Research, Innovation and National Laboratories to support research program development across campus, incorporating the combined research strengths of Argonne, Fermilab and the Marine Biological Laboratory.  She currently serves as the Chair of the Faculty Advisory Committee of the UChicago Center in Hong Kong. Her research interests are in the area of biophysics, and her laboratory is engaged in several projects, examining the differential recognition of phosphatidylserine by immnoreceptors, the membrane sealing capabilities of poloxamers, the targeting selectivity of antimicrobial peptides, the role of cholesterol in lipid ordering in membrane (lipid rafts), the interactions of lung surfactant peptides and lipids, and biomimetic materials. She was a Searle Scholar, a Packard Fellow and a Sloan Research Fellow. Ka Yee obtained her Sc.B. in Electrical Engineering from Brown University, her M.S. and Ph.D. in Applied Physics from Harvard University. 

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