Research program title
Engineering T-cells using microfluidics
Preliminary clinical trials harnessing the immune system of cancer patients to combat disease have shown to have impressive efficacy. Immune cells isolated from a patient are genetically altered to allow for improved detection of cancerous cells and subsequently re-introduced into the patient. Yet despite its promise, rapid and cost-effective manufacturing of this therapy remains a barrier to widespread adoption. The goal of this project is to implement a lab-on-a-chip approach to rapidly engineer CAR-T cells for cancer therapies. To achieve this goal, this work will be divided into three aims: 1) Design a microfluidic platform to deliver chimeric antigen receptors into model T-cells (see publication for preliminary work of gene-editing), 2) Activate and expansion of T-cells that are successfully transfected with CAR. 3) Perform the same protocols for primary and stem cells in collaboration with clinicians at St. Justine. If successful, this chip will allow for automated liquid handling and optimization of experimental conditions specific to the genetic engineering of T cells.
Academic qualifications required
PhD in Bioengineering or Electrical Engineering, experience in microfluidics and fabrication and synthetic biology an asset.